Diagnostic and Interventional Radiology
Abdominal Imaging - Original Article

In vivo and ex vivo measurements: noninvasive assessment of alcoholic fatty liver using 1H-MR spectroscopy

1.

Department of Diagnostic and Interventional Radiology , Johannes Wolfgang Goethe University Frankfurt, Frankfurt, Germany; These authors contributed equally to this work

2.

Department of Diagnostic and Interventional Radiology , Johannes Wolfgang Goethe University Frankfurt, Frankfurt, Germany

3.

Department of Biochemistry, Johannes Wolfgang Goethe University Frankfurt, Frankfurt, Germany

Diagn Interv Radiol 2016; 22: 13-21
DOI: 10.5152/dir.2015.14331
Read: 846 Downloads: 239 Published: 03 September 2019

Abstract

PURPOSE

We aimed to evaluate the ability of 1H-magnetic resonance spectroscopy (1H-MRS) to detect and quantify hepatic fat content in vivo and ex vivo in an experimental rat model of alcoholic fatty liver using histopathology, biochemistry, and laboratory analyses as reference.

 

METHODS

Alcoholic fatty liver was induced within 48 hours in 20 Lewis rats; 10 rats served as control. Intrahepatic fat content determined by 1H-MRS was expressed as the percent ratio of the lipid and water peaks and was correlated with intrahepatic fat content determined histologically and biochemically. Liver enzymes were measured in serum.

 

RESULTS

Fatty liver could be detected in vivo as well as ex vivo using 1H-MRS, in all 20 animals. Histologic analysis showed a fatty liver in 16 of 20 animals. Histology and 1H-MRS results were highly correlated (in vivo, r=0.93, P = 0.0005; ex vivo, r=0.92, P = 0.0006). Also a strong correlation was noted between in vivo 1H-MRS measurements and the fat content determined biochemically (r=0.96, P = 0.0003). Ex vivo results showed a similarly strong correlation between 1H-MRS and biochemistry (r=0.89, P = 0.0011).

 

CONCLUSION

1H-MRS can be carried out in ex vivo models, as well as in vivo, to detect and quantify intrahepatic fat content in the acute fatty liver.

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