Diagnostic and Interventional Radiology
Interventional Radiology - Original Article

Splenic artery embolization with Ankaferd blood stopper in a sheep model


Department of Radiology, Necmettin Erbakan University, Meram School of Medicine, Konya, Turkey


Department of Hematology, Gazi University School of Medicine, Ankara, Turkey


Department of Radiology, Selcuk University School of Medicine, Konya, Turkey


Department of Radiology, Afyon Kocatepe University School of Veterinary Medicine, Afyonkarahisar, Turkey


Department of Anesthesiology and Reanimation, Necmettin Erbakan University, Meram School of Medicine , Konya, Turkey


Department of Nephrology, Sakarya University Research and Training School of Medicine, Hospital, Sakarya, Turkey


Department of Pathology, Necmettin Erbakan University, Meram School of Medicine, Konya, Turkey


Department of Radiology, Mevlana University School of Medicine, Konya, Turkey


Department of Extracorporeal Hemocorrection, National Scientific Medical Research Center, Astana, Kazakhstan


Department of Hematology, Hacettepe University School of Medicine, Ankara, Turkey

Diagn Interv Radiol 2016; 22: 354-357
DOI: 10.5152/dir.2015.15382
Read: 534 Downloads: 144 Published: 03 September 2019


PURPOSE: Splenic artery embolization is a minimally invasive therapeutic procedure utilized in a number of disorders. Ankaferd blood stopper (ABS) is a novel hemostatic agent with a new mechanism of action independent of clotting factors. We aimed to investigate the safety and efficiency of ABS for splenic artery embolization in a sheep model.


METHODS: Seven adult female sheep were included in the study. Selective celiac angiography was performed using a 5F diagnostic catheter and then a 2.7F hydrophilic coating microcatheter was advanced coaxially to the distal part of the main splenic artery. Under fluoroscopic guidance, 6 mL mixture composed of half-and-half ABS and contrast agent was slowly injected. Fluoroscopy was used to observe the deceleration and stagnation of the flow. Control celiac angiograms were obtained immediately after the embolization. After the procedure, the animals were observed for one day and then sacrificed with intravenous sodium thiopental.


RESULTS: Technical success rate was 100%. None of the animals died or experienced a major systemic adverse event during the procedure. All of the spleens appeared dark on macroscopic examination due to excessive thrombosis. Microscopically, the majority of the splenic sinusoids (90%–95%) were necrotic.


CONCLUSION: In our study, splenic artery embolization by ABS was found to be safe and effective in the short-term. Further studies are needed to better understand the embolizing potential of this novel hemostatic agent.

EISSN 1305-3612